autofluorescence (Vector Laboratories)
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Vector Laboratories
autofluorescence
Autofluorescence, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trueview+autofluorescence+quenching+kit/Vector%C2%AE+TrueVIEW%C2%AE+Autofluorescence+Quenching+Kit/pmc12919959-231-1-4
Average 99 stars, based on 904 article reviews
Autofluorescence, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trueview+autofluorescence+quenching+kit/Vector%C2%AE+TrueVIEW%C2%AE+Autofluorescence+Quenching+Kit/pmc12919959-231-1-4
Average 99 stars, based on 904 article reviews
autofluorescence - by Bioz Stars,
2026-09
99/100 stars
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Blocking Assay:Article Title: Endothelial ACKR1 expression regulates neutrophil infiltration and breast cancer metastatic engraftment in the lung metastatic niche Article Snippet: .. The next day, sections were washed three times in PBS-T, treated with secondary antibodies (1:1000 in blocking buffer) for 1 hour at room temperature, and washed again in PBS-T. Quenching was performed with the Vector Fluorescence:Article Title: Micro/nanoplastics induce thyroid follicular cell pyroptosis to trigger thyrotoxicity by activating NF-κB signaling. Article Snippet: .. Nonspecific fluorescence was quenched using Vector® Article Title: Micro/nanoplastics induce thyroid follicular cell pyroptosis to trigger thyrotoxicity by activating NF-κB signaling Article Snippet: .. Nonspecific fluorescence was quenched using Vector Incubation:Article Title: HIF-1α + CD4 + T cells coordinate a tissue-resident immune cell network in the lung. Article Snippet: Lungs were washed in PBS, incubated in a 30% sucrose solution for 36 hours and embedded in OCT. For immunostaining of lung sections from Egln3-YFP mice, tissues were sectioned at 10-30 μm on a cryostat and incubated with 5% blocking buffer (Jackson ImmunoResearch) for 2 hours. .. Slides were incubated with indicated antibodies overnight at 4 ◦ C followed by washing with PBS, DAPI staining (2 μg/mL, Merck) and treatment with Staining:Article Title: HIF-1α + CD4 + T cells coordinate a tissue-resident immune cell network in the lung. Article Snippet: Lungs were washed in PBS, incubated in a 30% sucrose solution for 36 hours and embedded in OCT. For immunostaining of lung sections from Egln3-YFP mice, tissues were sectioned at 10-30 μm on a cryostat and incubated with 5% blocking buffer (Jackson ImmunoResearch) for 2 hours. .. Slides were incubated with indicated antibodies overnight at 4 ◦ C followed by washing with PBS, DAPI staining (2 μg/mL, Merck) and treatment with Article Title: The chromatin remodeling complex PRC2 safeguards cell fate in alveolar epithelial type 2 cells Article Snippet: Tissue sections were incubated with primary antibodies overnight at 4 degrees C followed by either a two-hour incubation at room temperature with fluorescently conjugated secondary antibodies or amplification using the ImmPRESS HRP Horse anti-Rabbit IgG Polymer Detection Kit (Vector Labs (MP-7401-50) and subsequent application of TSA fluorophores (Akoya Biosciences) (described in ). .. |
![Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei <t>(DAPI;</t> blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0342/pmc12810342/pmc12810342__gr3.jpg)